GSM4490307: d0_PY_3; Mus musculus; RNA-Seq - SRA

SRX8154495: GSM4490307: d0_PY_3; Mus musculus; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 14.5M spots, 1.1G bases, 424.3Mb downloads

Submitted by: NCBI (GEO)

Study: Remodeling of white fat during browning involves YBX1 to drive thermogenic commitment

PRJNA627345 • SRP257862 • All experiments • All runs

show Abstracthide Abstract

Effects of YBX1 activation in PPAR?-indcuded C3H/10T1/2-SAM pre-adipocytes on the transcriptome of cells during early differentation stages Overall design: C3H/10T1/2-SAM pre-adipocytes containing dCas9-VP64 and MS2-P65-HSF1 were transfected with guide RNA taregting PPAR? or a combination of guides taregting PPAR? and YBX1 two days before differentiation start to model gain of funtion in pre-adipocytes. Transcriptome was analysed for day 0, day 2 and day 4 of differentiation.

Sample: d0_PY_3

SAMN14668068 • SRS6516792 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Cells were lysed with RLT/β-Me and mRNA extracted using Rneasy spin columns (Quiagen) with DNAse treaamnet. mRNA concentrations were determined with nano-drop and RNA quality/integrity assessed using RNA 6000 Nano (Aligent Technology) chips. Illumina TruSeq Stranded Total RNA with Ribo-Zero Gold

Experiment attributes:

GEO Accession: GSM4490307

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 14.5M spots, 1.1G bases, 424.3Mb

Run	# of Spots	# of Bases	Size	Published
SRR11586944	14,512,161	1.1G	424.3Mb	2021-01-11

ID:: 10631728

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